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Supercritical Fluid Extraction: A Green Method for the Isolation of Valuable Substances from the Various Biological Matrices.
Topiař, Martin ; Sajfrtová, Marie ; Šolcová, Olga ; Hradecký, J. ; Hůrková, K. ; Hajšlová, J.
Supercritical fluid extraction (SFE) using carbon dioxide is a sophisticated technology suitable for isolation of un-polar, high value biological active substances from the various biological matrices. In this study, it was used for the extraction of Magnolia x pruhoniciana flowers and Japonochytrium marinum fungus. Results obtained from the SFE were compared with those from conventional extraction methods. A total number of 37 compounds were identified in the magnolia isolate, from which α-pinene (18 % of peak area), cymene (14 % of peak area), β-pinene (13 % of peak area) and humulene (6 % of peak area) were its main components. Yield of valuable free fatty acids DHA and EPA obtained by the SFE of Japonochytrium marinum was more than 1.5 times higher in comparison with organic liquid extraction using mixture of methanol and dichloromethane.
Plný tet: SKMBT_22317120815262 - Stáhnout plný textPDF
Plný text: content.csg - Stáhnout plný textPDF

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